An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis.

ABSTRACT: A systems-level understanding of a small but essential population of cells in development or adulthood (e.g. somatic stem cells) requires accurate quantitative monitoring of genome-wide gene expression, ideally from single cells. We report here a strategy to globally amplify mRNAs from single cells for highly quantitative high-density oligonucleotide microarray analysis that combines a small number of directional PCR cycles with subsequent linear amplification. Using this strategy, both the representation of gene expression profiles and reproducibility between individual experiments are unambiguously improved from the original method, along with high coverage and accuracy. The immediate application of this method to single cells in the undifferentiated inner cell masses of mouse blastocyst

SUBMITTER: Kurimoto K 

PROVIDER: S-EPMC1409679 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature