Multiplex degenerate PCR coupled with an oligo sorbent array for human endogenous retrovirus expression profiling.

ABSTRACT: Human endogenous retroviruses (HERVs) can be divided into distinct families of tens to thousands of paralogous loci. The expression of HERV elements has been detected in all tissues tested to date, particularly germ cells, embryonic tissues and neoplastic tissues. Hence, the study of HERV expression could represent added value in cancer diagnosis. We developed a quantitative assay combining a multiplex degenerate PCR (MD-PCR) amplification, based on the relative conservation of the pol genes, and a colorimetric Oligo Sorbent Array (OLISA). Nine HERV families were selected and amplification primers and capture probes were designed for each family. The features required to achieve efficient amplification of most of the elements of each HERV family and balanced co-amplification of all HERV fa

SUBMITTER: Pichon JP 

PROVIDER: S-EPMC1409818 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature