Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice.

ABSTRACT:

Background

Lim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at the nephric vesicle stage, leading to the production of small, non-functional kidneys that lack nephrons.

Methods

In the present study, we used Affymetrix probe arrays to screen for nephron-specific genes by comparing the expression profiles of control and Lim1 conditional mutant kidneys. Kidneys from two developmental stages, embryonic day 14.5 (E14.5) and 18.5 (E18.5), were examined.

Results

Comparison of E18.5 kidney expression profiles generated a list of 465 nephron-sp

SUBMITTER: Chen YT 

PROVIDER: S-EPMC1413522 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature