Molecular targets for rapid identification of Brucella spp.

ABSTRACT:

Background

Brucella is an intracellular pathogen capable of infecting animals and humans. There are six recognized species of Brucella that differ in their host preference. The genomes of the three Brucella species have been recently sequenced. Comparison of the three revealed over 98% sequence similarity at the protein level and enabled computational identification of common and differentiating genes. We validated these computational predictions and examined the expression patterns of the putative unique and differentiating genes, using genomic and reverse transcription PCR. We then screened a set of differentiating genes against classical Brucella biovars and showed the applicability of these regions in the design of diagnostic tests.

Results

We have identified and tested

SUBMITTER: Ratushna VG 

PROVIDER: S-EPMC1413539 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature