Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus subtilis.

ABSTRACT: The regulation of initiation of DNA replication is crucial to ensure that the genome is replicated only once per cell cycle. In the Gram-positive bacterium Bacillus subtilis, the function of the YabA protein in initiation control was assigned based on its interaction with the DnaA initiator and the DnaN sliding clamp in the yeast two-hybrid and on the overinitiation phenotype observed in a yabA null strain. However, YabA is unrelated to known regulators of initiation and interacts with several additional proteins that could also be involved directly or not in initiation control. Here, we investigated the specific role of YabA interactions with DnaA and DnaN in initiation control by identifying single amino acid changes in YabA that disrupted solely the interaction with DnaA or DnaN. These

SUBMITTER: Noirot-Gros MF 

PROVIDER: S-EPMC1413692 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature