Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells.

ABSTRACT: Infections with hepatitis C virus (HCV) are marked by frequent viral persistence, chronic liver disease, and extraordinary viral genetic diversity. Although much has been learned about HCV since its discovery, progress has been slowed by a lack of permissive cell culture systems supporting its replication. Productive infections have been achieved recently with genotype 2a virus, but cirrhosis and liver cancer are typically associated with genotype 1 HCV, which is more prevalent and relatively resistant to IFN therapy. We describe production of infectious genotype 1a HCV in cells transfected with synthetic RNA derived from a prototype virus (H77-S). Viral proteins accumulated more slowly in H77-S transfected cells than in cells transfected with genotype 2a (JFH-1) RNA, but substantially mor

SUBMITTER: Yi M 

PROVIDER: S-EPMC1413728 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature