An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants.

ABSTRACT: Random transposon insertion libraries have proven invaluable in studying bacterial genomes. Libraries that approach saturation must be large, with multiple insertions per gene, making comprehensive genome-wide scanning difficult. To facilitate genome-scale study of the opportunistic human pathogen Pseudomonas aeruginosa strain PA14, we constructed a nonredundant library of PA14 transposon mutants (the PA14NR Set) in which nonessential PA14 genes are represented by a single transposon insertion chosen from a comprehensive library of insertion mutants. The parental library of PA14 transposon insertion mutants was generated by using MAR2xT7, a transposon compatible with transposon-site hybridization and based on mariner. The transposon-site hybridization genetic footprinting feature broadens

SUBMITTER: Liberati NT 

PROVIDER: S-EPMC1413827 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature