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Rhodopsin self-associates in asolectin liposomes.

ABSTRACT: We show that the photoreceptor rhodopsin (Rh) can exist in the membrane as a dimer or multimer using luminescence resonance energy transfer and FRET methods. Our approach looked for interactions between Rh molecules reconstituted into asolectin liposomes. The low receptor density used in the measurements ensured minimal receptor crowding and artifactual association. The fluorescently labeled Rh molecules were fully functional, as measured by their ability to activate the G protein transducin. The luminescence resonance energy transfer measurements revealed a distance of 47-50 Angstroms between Rh molecules. The measured efficiency of FRET between receptors was close to the theoretical maximum possible, indicating nearly quantitative Rh-Rh association. Together, these results provide compelling evidence that Rh spontaneously self-associates in membranes.

SUBMITTER: Mansoor SE

PROVIDER: S-EPMC1413906 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

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Rhodopsin self-associates in asolectin liposomes.

Mansoor Steven E SE Palczewski Krzysztof K Farrens David L DL

Proceedings of the National Academy of Sciences of the United States of America 20060221 9

We show that the photoreceptor rhodopsin (Rh) can exist in the membrane as a dimer or multimer using luminescence resonance energy transfer and FRET methods. Our approach looked for interactions between Rh molecules reconstituted into asolectin liposomes. The low receptor density used in the measurements ensured minimal receptor crowding and artifactual association. The fluorescently labeled Rh molecules were fully functional, as measured by their ability to activate the G protein transducin. Th ...[more]

PMID: 16492772

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