Defining the primary route for lutein synthesis in plants: the role of Arabidopsis carotenoid beta-ring hydroxylase CYP97A3.

ABSTRACT: Lutein, a dihydroxy derivative of alpha-carotene (beta,epsilon-carotene), is the most abundant carotenoid in photosynthetic plant tissues where it plays important roles in light-harvesting complex-II structure and function. The synthesis of lutein from lycopene requires at least four distinct enzymatic reactions: beta- and epsilon-ring cyclizations and hydroxylation of each ring at the C-3 position. Three carotenoid hydroxylases have already been identified in Arabidopsis, two nonheme diiron beta-ring monooxygenases (the B1 and B2 loci) that primarily catalyze hydroxylation of the beta-ring of beta,beta-carotenoids and one heme-containing monooxygenase (CYP97C1, the LUT1 locus) that catalyzes hydroxylation of the epsilon-ring of beta,epsilon-carotenoids. In this study, we demonstrate that

SUBMITTER: Kim J 

PROVIDER: S-EPMC1413914 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature