Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3.

ABSTRACT:

Background

Although 2,061 proteins of Pyrococcus horikoshii OT3, a hyperthermophilic archaeon, have been predicted from the recently completed genome sequence, the majority of proteins show no similarity to those from other organisms and are thus hypothetical proteins of unknown function. Because most proteins operate as parts of complexes to regulate biological processes, we systematically analyzed protein-protein interactions in Pyrococcus using the mammalian two-hybrid system to determine the function of the hypothetical proteins.

Results

We examined 960 soluble proteins from Pyrococcus and selected 107 interactions based on luciferase reporter activity, which was then evaluated using a computational approach to assess the reliability of the interactions. We also analyzed

SUBMITTER: Usui K 

PROVIDER: S-EPMC1414084 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature