Identification, cloning and functional characterization of novel beta-defensins in the rat (Rattus norvegicus).

ABSTRACT:

Background

Beta-defensins are small cationic peptides that exhibit broad spectrum antimicrobial properties. The majority of beta-defensins identified in humans are predominantly expressed in the male reproductive tract and have roles in non-immunological processes such as sperm maturation and capacitation. Characterization of novel defensins in the male reproductive tract can lead to increased understanding of their dual roles in immunity and sperm maturation.

Methods

In silico rat genomic analyses were used to identify novel beta-defensins related to human defensins 118-123. RNAs isolated from male reproductive tract tissues of rat were reverse transcribed and PCR amplified using gene specific primers for defensins. PCR products were sequenced to confirm their identity. RT-

SUBMITTER: Yenugu S 

PROVIDER: S-EPMC1420305 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature