Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites.

ABSTRACT:

Background

Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In the Creator system, an ORF introduced into a donor vector can be transferred with Cre recombinase to a library of acceptor vectors optimized for different applications. Usability of the Creator system is impacted by the ability to easily manipulate DNA, the number of acceptor vectors for downstream applications, and the level of protein expression from Creator vectors.

Results

To date, we have developed over 20 novel acceptor vectors that employ a variety of promoters and epitope tags commonly employed for proteomics applications and gene function analysis. We

SUBMITTER: Colwill K 

PROVIDER: S-EPMC1421398 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature