Identification of glycosylated marker proteins of epithelial polarity in MDCK cells by homology driven proteomics.

ABSTRACT:

Background

MDCK cells derived from canine kidney are an important experimental model system for investigating epithelial polarity in mammalian cells. Monoclonal antibodies against apical gp114 and basolateral p58 have served as important tools in these studies. However, the molecular identity of these membrane glycoproteins has not been known.

Results

We have identified the sialoglycoprotein gp114 as a dog homologue of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family. Gp114 was enriched from tissue culture cells by subcellular fractionation and immunoaffinity chromatography. The identification was based on tandem mass spectrometry and homology based proteomics. In addition, the p58 basolateral marker glycoprotein was found to be the beta subunit of

SUBMITTER: Fullekrug J 

PROVIDER: S-EPMC1421407 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature