Selection of antisense oligonucleotides based on multiple predicted target mRNA structures.

ABSTRACT:

Background

Local structures of target mRNAs play a significant role in determining the efficacies of antisense oligonucleotides (ODNs), but some structure-based target site selection methods are limited by uncertainties in RNA secondary structure prediction. If all the predicted structures of a given mRNA within a certain energy limit could be used simultaneously, target site selection would obviously be improved in both reliability and efficiency. In this study, some key problems in ODN target selection on the basis of multiple predicted target mRNA structures are systematically discussed.

Results

Two methods were considered for merging topologically different RNA structures into integrated representations. Several parameters were derived to characterize local target site s

SUBMITTER: Bo X 

PROVIDER: S-EPMC1421440 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature