Microarray based comparison of two Escherichia coli O157:H7 lineages.

ABSTRACT:

Background

Previous research has identified the potential for the existence of two separate lineages of Escherichia coli O157:H7. Clinical isolates tended to cluster primarily within one of these two lineages. To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening.

Results

Using a 610 gene microarray, designed against the E. coli O157 EDL 933 transcriptome, targeting primarily virulence systems, we chose 3 representative Lineage I isolates (LI groups mostly clinical isolates) and 3 representative Lineage II isolates (LII groups mostly bovine isolates). Using standard dye swap experimental designs, statistically different expression (P < 0.05) of 73 genes bet

SUBMITTER: Dowd SE 

PROVIDER: S-EPMC1431545 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature