Molecular alterations in primary prostate cancer after androgen ablation therapy.

ABSTRACT:

Purpose

After an initial response to androgen ablation, most prostate tumors recur, ultimately progressing to highly aggressive androgen-independent cancer. The molecular mechanisms underlying progression are not well known in part due to the rarity of androgen-independent samples from primary and metastatic sites.

Experimental design

We compared the gene expression profiles of 10 androgen-independent primary prostate tumor biopsies with 10 primary, untreated androgen-dependent tumors. Samples were laser capture microdissected, the RNA was amplified, and gene expression was assessed using Affymetrix Human Genome U133A GeneChip. Differential expression was examined with principal component analysis, hierarchical clustering, and Student's t testing. Analysis of gene ontology w

SUBMITTER: Best CJ 

PROVIDER: S-EPMC1432092 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature