Conformational transitions in protein-protein association: binding of fasciculin-2 to acetylcholinesterase.

ABSTRACT: The neurotoxin fasciculin-2 (FAS2) is a picomolar inhibitor of synaptic acetylcholinesterase (AChE). The dynamics of binding between FAS2 and AChE is influenced by conformational fluctuations both before and after protein encounter. Submicrosecond molecular dynamics trajectories of apo forms of fasciculin, corresponding to different conformational substates, are reported here with reference to the conformational changes of loop I of this three-fingered toxin. This highly flexible loop exhibits an ensemble of conformations within each substate corresponding to its functions. The high energy barrier found between the two major substates leads to transitions that are slow on the timescale of the diffusional encounter of noninteracting FAS2 and AChE. The more stable of the two apo substates ma

SUBMITTER: Bui JM 

PROVIDER: S-EPMC1432132 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature