Impact of vector priming on the immunogenicity of recombinant Salmonella vaccines.

ABSTRACT: There are conflicting reports concerning the impact of prior vector priming on the immunogenicity of recombinant-Salmonella-based vaccines. A comparison of experimental protocols identified two variables which might account for this inconsistency: the potential of the vector strain to colonize the murine gut-associated lymphoid tissue (GALT) and the nature of the foreign antigen subsequently delivered by the recombinant Salmonella construct. The former was investigated by constructing an aroA mutant of the Salmonella enterica serovar Stanley vector previously used in our laboratory. Although the introduction of an aroA mutation had surprisingly little effect on GALT colonization, it did reduce the strength of antilipopolysaccharide (anti-LPS) antibody responses and the impact of vector pri

SUBMITTER: Vindurampulle CJ 

PROVIDER: S-EPMC143414 | biostudies-literature | 2003 Jan

REPOSITORIES: biostudies-literature