Cell type-specific over-expression of chromosome 21 genes in fibroblasts and fetal hearts with trisomy 21.

ABSTRACT:

Background

Down syndrome (DS) is caused by trisomy 21 (+21), but the aberrations in gene expression resulting from this chromosomal aneuploidy are not yet completely understood.

Methods

We used oligonucleotide microarrays to survey mRNA expression in early- and late-passage control and +21 fibroblasts and mid-gestation fetal hearts. We supplemented this analysis with northern blotting, western blotting, real-time RT-PCR, and immunohistochemistry.

Results

We found chromosome 21 genes consistently over-represented among the genes over-expressed in the +21 samples. However, these sets of over-expressed genes differed across the three cell/tissue types. The chromosome 21 gene MX1 was strongly over-expressed (mean 16-fold) in senescent +21 fibroblasts, a result verified by

SUBMITTER: Li CM 

PROVIDER: S-EPMC1435874 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature