Characterization of functional domains of equine infectious anemia virus Rev suggests a bipartite RNA-binding domain.

ABSTRACT: Equine infectious anemia virus (EIAV) Rev is an essential regulatory protein that facilitates expression of viral mRNAs encoding structural proteins and genomic RNA and regulates alternative splicing of the bicistronic tat/rev mRNA. EIAV Rev is characterized by a high rate of genetic variation in vivo, and changes in Rev genotype and phenotype have been shown to coincide with changes in clinical disease. To better understand how genetic variation alters Rev phenotype, we undertook deletion and mutational analyses to map functional domains and to identify specific motifs that are essential for EIAV Rev activity. All functional domains are contained within the second exon of EIAV Rev. The overall organization of domains within Rev exon 2 includes a nuclear export signal, a large central regi

SUBMITTER: Lee JH 

PROVIDER: S-EPMC1440447 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature