Comparison of gene coverage of mouse oligonucleotide microarray platforms.

ABSTRACT:

Background

The increasing use of DNA microarrays for genetical genomics studies generates a need for platforms with complete coverage of the genome. We have compared the effective gene coverage in the mouse genome of different commercial and noncommercial oligonucleotide microarray platforms by performing an in-house gene annotation of probes. We only used information about probes that is available from vendors and followed a process that any researcher may take to find the gene targeted by a given probe. In order to make consistent comparisons between platforms, probes in each microarray were annotated with an Entrez Gene id and the chromosomal position for each gene was obtained from the UCSC Genome Browser Database. Gene coverage was estimated as the percentage of Entrez Genes w

SUBMITTER: Verdugo RA 

PROVIDER: S-EPMC1440853 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature