Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis.

ABSTRACT:

Background

Brucella abortus is a facultative intracellular pathogen that mainly infects cattle and humans. Current vaccines rely on live attenuated strains of B. abortus, which can revert to their pathogenic status and thus are not totally safe for use in humans. Therefore, the development of mucosal live vaccines using the food-grade lactic acid bacterium, Lactococcus lactis, as an antigen delivery vector, is an attractive alternative and a safer vaccination strategy against B. abortus. Here, we report the construction of L. lactis strains genetically modified to produce B. abortus GroEL heat-shock protein, a candidate antigen, in two cellular locations, intracellular or secreted.

Results

Only the secreted form of GroEL was stably produced in L. lactis, suggesting a detrime

SUBMITTER: Miyoshi A 

PROVIDER: S-EPMC1444932 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature