The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.

ABSTRACT: This study describes the characterization of the riboflavin transport protein RibU in the lactic acid bacterium Lactococcus lactis subsp. cremoris NZ9000. RibU is predicted to contain five membrane-spanning segments and is a member of a novel transport protein family, not described in the Transport Classification Database. Transcriptional analysis revealed that ribU transcription is downregulated in response to riboflavin and flavin mononucleotide (FMN), presumably by means of the structurally conserved RFN (riboflavin) element located between the transcription start site and the start codon. An L. lactis strain carrying a mutated ribU gene exhibits altered transcriptional control of the riboflavin biosynthesis operon ribGBAH in response to riboflavin and FMN and does not consume riboflavi

SUBMITTER: Burgess CM 

PROVIDER: S-EPMC1446998 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature