RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics.

ABSTRACT: Legionella pneumophila translocates multiple bacterial effector proteins into host cells to direct formation of a replication vacuole for the bacterium. The emerging consensus is that formation of this compartment involves recruitment of membrane material that traffics between the endoplasmic reticulum (ER) and Golgi. To investigate this model, a targeted approach was used to knock down expression of proteins involved in membrane trafficking, using RNA interference in Drosophila cells. Surprisingly, few single knockdowns of ER-Golgi transport proteins decreased L. pneumophila replication. By analyzing double-stranded RNAs in pairs, combinations were identified that together caused defects in intracellular replication, consistent with the model that membrane traffic funnels into the replica

SUBMITTER: Dorer MS 

PROVIDER: S-EPMC1447669 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature