Movies of cellular and sub-cellular motion by digital holographic microscopy.

ABSTRACT:

Background

Many biological specimens, such as living cells and their intracellular components, often exhibit very little amplitude contrast, making it difficult for conventional bright field microscopes to distinguish them from their surroundings. To overcome this problem phase contrast techniques such as Zernike, Normarsky and dark-field microscopies have been developed to improve specimen visibility without chemically or physically altering them by the process of staining. These techniques have proven to be invaluable tools for studying living cells and furthering scientific understanding of fundamental cellular processes such as mitosis. However a drawback of these techniques is that direct quantitative phase imaging is not possible. Quantitative phase imaging is important becau

SUBMITTER: Mann CJ 

PROVIDER: S-EPMC1448199 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature