"Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays.

ABSTRACT:

Background

Transcriptome data from quantitative PCR (Q-PCR) and DNA microarrays are typically obtained from a fixed amount of RNA collected per sample. Therefore, variations in tissue cellularity and RNA yield across samples in an experimental series compromise accurate determination of the absolute level of each mRNA species per cell in any sample. Since mRNAs are copied from genomic DNA, the simplest way to express mRNA level would be as copy number per template DNA, or more practically, as copy number per cell.

Results

Here we report a method (designated the "Percellome" method) for normalizing the expression of mRNA values in biological samples. It provides a "per cell" readout in mRNA copy number and is applicable to both quantitative PCR (Q-PCR) and DNA microarray stud

SUBMITTER: Kanno J 

PROVIDER: S-EPMC1448209 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature