Use of TaqMan real-time reverse transcription-PCR for rapid detection, quantification, and typing of norovirus.

ABSTRACT: Noroviruses (NoVs) are the most commonly identified cause of outbreaks and sporadic cases of acute gastroenteritis. We evaluated and optimized NoV-specific TaqMan real-time reverse transcription (RT)-PCR assays for the rapid detection and typing of NoV strains belonging to genogroups GI and GII and adapted them to the LightCycler platform. We expanded the detection ability of the assays by developing an assay that detects the GIV NoV strain. The assays were validated with 92 clinical samples and 33 water samples from confirmed NoV outbreaks and suspected NoV contamination cases. The assays detected NoV RNA in all of the clinical specimens previously confirmed positive by conventional RT-PCR and sequencing. Additionally, the TaqMan assays successfully detected NoV RNA in water samples conta

SUBMITTER: Trujillo AA 

PROVIDER: S-EPMC1448641 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature