Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array.

ABSTRACT: To accurately determine the pathotypes of Escherichia coli strains, a comprehensive assessment of each strain that targets multiple genes is required. A new approach to the identification and characterization of E. coli pathotypes was developed by constructing gene-specific probes (70-mers) for not only the virulence genes associated with each E. coli pathotype but also the O157-, CFT073-, and K-12-specific and common genes of each pathotype. Analysis of oligonucleotide probes with reference and clinical isolates of E. coli pathotypes indicated that the array could differentiate the pathotypes on the basis of their virulence and specific gene patterns. Probes targeting common genes of E. coli were present in all the reference and clinical strains. Salmonella enterica subsp. enterica-specif

SUBMITTER: Palaniappan RU 

PROVIDER: S-EPMC1448672 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature