Forty-eight-hour diagnosis of onychomycosis with subtyping of Trichophyton rubrum strains.

ABSTRACT: A novel strategy for the molecular identification of fungal agents of onychomycosis (including Trichophyton rubrum) has been designed based on the use of species-specific and universal primers in conjunction with a commercial kit that allows the extraction of DNA directly from the nail specimens. The microsatellite marker T1, which is based on a (GT)n repeat, was applied for the species-specific identification of Trichophyton rubrum. To evaluate how often Scopulariopsis spp. are detected in nail specimens, a second primer pair was designed to amplify specifically a 336-bp DNA fragment of the 28S region of the nuclear rRNA gene of S. brevicaulis and closely related species. Other fungal species were identified using amplification of the internal transcribed spacer (ITS) region of the rRNA g

SUBMITTER: Kardjeva V 

PROVIDER: S-EPMC1448676 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature