Organ-specific silencing of duplicated genes in a newly synthesized cotton allotetraploid.

ABSTRACT: Most eukaryotes have undergone genome doubling at least once during their evolutionary history. Hybridization followed by genome doubling (allopolyploidization) is a prominent mode of speciation in plants, leading to phenotypic novelty and changes in genome structure and gene expression. Molecular events that take place immediately after polyploid formation can be studied using newly synthesized allopolyploids. Here we studied the extent of gene silencing in a newly created and genomically stable allotetraploid cotton, of genotype AAGG, using an AFLP-cDNA display screen. Over 2000 transcripts were screened and approximately 5% of the duplicated genes in the allotetraploid were inferred to have been silenced or downregulated. Sequencing of 24 AFLP-cDNA fragments revealed genes with a variet

SUBMITTER: Adams KL 

PROVIDER: S-EPMC1448729 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature