Mutations in Su(var)205 and Su(var)3-7 suppress P-element-dependent silencing in Drosophila melanogaster.

ABSTRACT: In Drosophila melanogaster, the w(+) transgene in P[lacW]ci(Dplac) is uniformly expressed throughout the adult eye. However, when other P elements are present, this w(+) transgene is randomly silenced and this produces a variegated eye phenotype. This P-element-dependent silencing (PDS) is limited to w(+) transgenes inserted in a specific region on chromosome 4. In a screen for genetic modifiers of PDS, we isolated mutations in Su(var)205, Su(var)3-7, and two unidentified genes that suppress this variegated phenotype. Therefore, only a few of the genes encoding heterochromatic modifiers act dose dependently in PDS. In addition, we recovered two spontaneous mutations of P[lacW]ci(Dplac) that variegate in the absence of P elements. These P[lacW]i(Dplac) derivatives have a gypsy element inser

SUBMITTER: Bushey D 

PROVIDER: S-EPMC1448784 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature