The LF1 gene of Chlamydomonas reinhardtii encodes a novel protein required for flagellar length control.

ABSTRACT: Flagellar length is tightly regulated in the biflagellate alga Chlamydomonas reinhardtii. Several genes required for control of flagellar length have been identified, including LF1, a gene required to assemble normal-length flagella. The lf1 mutation causes cells to assemble extra-long flagella and to regenerate flagella very slowly after amputation. Here we describe the positional cloning and molecular characterization of the LF1 gene using a bacterial artificial chromosome (BAC) library. LF1 encodes a protein of 804 amino acids with no obvious sequence homologs in other organisms. The single LF1 mutant allele is caused by a transversion that produces an amber stop at codon 87. Rescue of the lf1 phenotype upon transformation was obtained with clones containing the complete LF1 gene as wel

SUBMITTER: Nguyen RL 

PROVIDER: S-EPMC1449559 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature