Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sites.

ABSTRACT: The chromosomes of the macronuclear (expressed) genome of Tetrahymena thermophila are generated by developmental fragmentation of the five micronuclear (germline) chromosomes. This fragmentation is site specific and directed by a conserved 15-bp chromosome breakage sequence (Cbs element). This article reports the construction of a library enriched for chromosome breakage junctions and the development of a successful scheme for the genome-wide isolation and characterization of functional Cbs junctions. Twenty-three new Cbs junctions were characterized and each was assigned to a specific micronuclear chromosome or chromosome arm. Two distinct previously unreported variant chromosome breakage sequences were found, each in two or more functional Cbs elements. Analysis of natural Cbs junctions

SUBMITTER: Hamilton E 

PROVIDER: S-EPMC1449750 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature