Genome-wide characterization of Tetrahymena thermophila chromosome breakage sites. II. Physical and genetic mapping.

ABSTRACT: The chromosomes of the macronuclear (expressed) genome of Tetrahymena thermophila are generated by developmental fragmentation of the five micronuclear (germline) chromosomes. This fragmentation is site specific, directed by a conserved chromosome breakage sequence (Cbs element). An accompanying article in this issue reports the development of a successful scheme for the genome-wide cloning and identification of functional chromosome breakage sites. This article reports the physical and genetic characterization of 30 functional chromosome breakage junctions. Unique sequence tags and physical sizes were obtained for the pair of macronuclear chromosomes generated by fragmentation at each Cbs. Cbs-associated polymorphisms were used to genetically map 11 junctions to micronuclear linkage group

SUBMITTER: Cassidy-Hanley D 

PROVIDER: S-EPMC1449751 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature