Differential effects of HOXB4 on nonhuman primate short- and long-term repopulating cells.

ABSTRACT:

Background

Hematopoietic stem cells (HSCs) or repopulating cells are able to self-renew and differentiate into cells of all hematopoietic lineages, and they can be enriched using the CD34 cell surface marker. Because of this unique property, HSCs have been used for HSC transplantation and gene therapy applications. However, the inability to expand HSCs has been a significant limitation for clinical applications. Here we examine, in a clinically relevant nonhuman primate model, the ability of HOXB4 to expand HSCs to potentially overcome this limitation.

Methods and findings

Using a competitive repopulation assay, we directly compared in six animals engraftment of HOXB4GFP (HOXB4 green fluorescent protein) and control (yellow fluorescent protein [YFP])-transduced and expanded

SUBMITTER: Zhang XB 

PROVIDER: S-EPMC1450018 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature