A phosphorylation map of the bovine papillomavirus E1 helicase.

ABSTRACT:

Background

Papillomaviruses undergo a complex life cycle requiring regulated DNA replication. The papillomavirus E1 helicase is essential for viral DNA replication and plays a key role in controlling viral genome copy number. The E1 helicase is regulated at least in part by protein phosphorylation, however no systematic approach to phosphate site mapping has been attempted. We have utilized mass spectrometry of purified bovine papillomavirus E1 protein to identify and characterize new sites of phosphorylation.

Results

Mass spectrometry and in silico sequence analysis were used to identify phosphate sites on the BPV E1 protein and kinases that may recognize these sites. Five new and two previously known phosphorylation sites were identified. A phosphate site map was created a

SUBMITTER: Lentz MR 

PROVIDER: S-EPMC1450263 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature