Gene expression profiling of naive sheep genetically resistant and susceptible to gastrointestinal nematodes.

ABSTRACT:

Background

Gastrointestinal nematodes constitute a major cause of morbidity and mortality in grazing ruminants. Individual animals or breeds, however, are known to differ in their resistance to infection. Gene expression profiling allows us to examine large numbers of transcripts simultaneously in order to identify those transcripts that contribute to an animal's susceptibility or resistance.

Results

With the goal of identifying genes with a differential pattern of expression between sheep genetically resistant and susceptible to gastrointestinal nematodes, a 20,000 spot ovine cDNA microarray was constructed. This array was used to interrogate the expression of 9,238 known genes in duodenum tissue of four resistant and four susceptible female lambs. Naïve animals were used i

SUBMITTER: Keane OM 

PROVIDER: S-EPMC1450279 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature