Structural diversity and differential transcription of the patatin multicopy gene family during potato tuber development.

ABSTRACT: The patatin multicopy gene family encodes the major storage protein in potato tubers and is organized as a single cluster in the potato genome. We sequenced a 154-kb bacterial artificial chromosome (BAC) clone containing a portion of the patatin gene cluster. Two putatively functional patatin genes were found in this BAC. These two genes are embedded within arrays of patatin pseudogenes. Using a chromatin immunoprecipitation method we demonstrate that the dramatic increase of patatin gene expression during the transition from stolons to tubers coincides with an increase of histone H4 lysine acetylation. We used 3' rapid amplification of cDNA ends to profile expression of different patatin genes during tuber development. The profiling results revealed differential expression patterns of spe

SUBMITTER: Stupar RM 

PROVIDER: S-EPMC1456224 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature