EST2Prot: mapping EST sequences to proteins.

ABSTRACT:

Background

EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked.

Results

We describe a system (EST2Prot) that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors.

Conclusion

SUBMITTER: Shafer P 

PROVIDER: S-EPMC1456965 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature