Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis.

ABSTRACT: Although advances in microfluidic technology have enabled increasingly sophisticated biosensing and bioassay operations to be performed at the microscale, many of these applications employ such small amounts of charged biomolecules (DNA, proteins, and peptides) that they must first be preconcentrated to a detectable level. Efficient strategies for precisely handling minute quantities of biomolecules in microchannel geometries are critically needed; however, it has proven challenging to achieve simultaneous concentration, focusing, and metering capabilities with current-generation sample-injection technology. By using microfluidic chips incorporating arrays of individually addressable microfabricated electrodes, we demonstrate that DNA can be sequentially concentrated, focused into a narrow

SUBMITTER: Shaikh FA 

PROVIDER: S-EPMC1458754 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature