Global gene expression analyses of hematopoietic stem cell-like cell lines with inducible Lhx2 expression.

ABSTRACT:

Background

Expression of the LIM-homeobox gene Lhx2 in murine hematopoietic cells allows for the generation of hematopoietic stem cell (HSC)-like cell lines. To address the molecular basis of Lhx2 function, we generated HSC-like cell lines where Lhx2 expression is regulated by a tet-on system and hence dependent on the presence of doxycyclin (dox). These cell lines efficiently down-regulate Lhx2 expression upon dox withdrawal leading to a rapid differentiation into various myeloid cell types.

Results

Global gene expression of these cell lines cultured in dox was compared to different time points after dox withdrawal using microarray technology. We identified 267 differentially expressed genes. The majority of the genes overlapping with HSC-specific databases were those down-

SUBMITTER: Richter K 

PROVIDER: S-EPMC1459142 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature