Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes.

ABSTRACT: Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.

SUBMITTER: Kelly S 

PROVIDER: S-EPMC1459678 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature