Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase.

ABSTRACT: Schizosaccharomyces pombe senses environmental glucose through a cAMP-signaling pathway, activating cAMP-dependent protein kinase A (PKA). This requires nine git (glucose insensitive transcription) genes that encode adenylate cyclase, the PKA catalytic subunit, and seven "upstream" proteins required for glucose-triggered adenylate cyclase activation, including three heterotrimeric G-protein subunits and its associated receptor. We describe here the cloning and characterization of the git1+ gene. Git1 is distantly related to a small group of uncharacterized fungal proteins, including a second S. pombe protein that is not functionally redundant with Git1, as well as to members of the UNC-13/Munc13 protein family. Mutations in git1+ demonstrate functional roles for the two most highly conserv

SUBMITTER: Kao RS 

PROVIDER: S-EPMC1461440 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature