Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro.

ABSTRACT:

Background

This study has investigated the ability of tobacco smoke, and ingredients of tobacco smoke, to induce apoptosis in the airway epithelial cell line A549.

Method

A549 cells were treated with 80 microg/ml Tobacco smoke condensate (TSC), 10 mM Nicotine, 10 microM paraldehyde, 10 microM hydrogen peroxide, 1 microM Taxol (Paclitaxel), 100%, 50% and 25% cigarette smoke extract (CSE). Following 4-48 h incubation apoptosis was measured morphologically following staining of cells with DAPI. TUNEL staining was also used to assess DNA damage after 24 and 48 h incubation. In addition, loss of mitochondrial cytochrome C and activation of Bax-alpha, early events in the apoptotic process, were measured after 4 h of incubation.

Results

Incubation of A549 cells with vehicle,

SUBMITTER: Ramage L 

PROVIDER: S-EPMC1462990 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature