Voltage sensor conformations in the open and closed states in ROSETTA structural models of K(+) channels.

ABSTRACT: Voltage-gated ion channels control generation and propagation of action potentials in excitable cells. Significant progress has been made in understanding structure and function of the voltage-gated ion channels, highlighted by the high-resolution open-state structure of the voltage-gated potassium channel, K(v)1.2. However, because the structure of the closed state is unknown, the gating mechanism remains controversial. We adapted the rosetta membrane method to model the structures of the K(v)1.2 and KvAP channels using homology, de novo, and domain assembly methods and selected the most plausible models using a limited number of experimental constraints. Our model of K(v)1.2 in the open state is very similar in overall topology to the x-ray structure of this channel. Modeling of KvAP in

SUBMITTER: Yarov-Yarovoy V 

PROVIDER: S-EPMC1464335 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature