Overflow metabolism in Escherichia coli during steady-state growth: transcriptional regulation and effect of the redox ratio.

ABSTRACT: Overflow metabolism in the form of aerobic acetate excretion by Escherichia coli is an important physiological characteristic of this common industrial microorganism. Although acetate formation occurs under conditions of high glucose consumption, the genetic mechanisms that trigger this phenomenon are not clearly understood. We report on the role of the NADH/NAD ratio (redox ratio) in overflow metabolism. We modulated the redox ratio in E. coli through the expression of Streptococcus pneumoniae (water-forming) NADH oxidase. Using steady-state chemostat cultures, we demonstrated a strong correlation between acetate formation and this redox ratio. We furthermore completed genome-wide transcription analyses of a control E. coli strain and an E. coli strain overexpressing NADH oxidase. The tra

SUBMITTER: Vemuri GN 

PROVIDER: S-EPMC1472329 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature