Chromosome-wide gene-specific targeting of the Drosophila dosage compensation complex.

ABSTRACT: The dosage compensation complex (DCC) of Drosophila melanogaster is capable of distinguishing the single male X from the other chromosomes in the nucleus. It selectively interacts in a discontinuous pattern with much of the X chromosome. How the DCC identifies and binds the X, including binding to the many genes that require dosage compensation, is currently unknown. To identify bound genes and attempt to isolate the targeting cues, we visualized male-specific lethal 1 (MSL1) protein binding along the X chromosome by combining chromatin immunoprecipitation with high-resolution microarrays. More than 700 binding regions for the DCC were observed, encompassing more than half the genes found on the X chromosome. In addition, several rare autosomal binding sites were identified. Essential gene

SUBMITTER: Gilfillan GD 

PROVIDER: S-EPMC1475731 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature