Nuclear mRNA degradation pathway(s) are implicated in Xist regulation and X chromosome inactivation.

ABSTRACT: A critical step in X-chromosome inactivation (XCI), which results in the dosage compensation of X-linked gene expression in mammals, is the coating of the presumptive inactive X chromosome by the large noncoding Xist RNA, which then leads to the recruitment of other factors essential for the heterochromatinisation of the inactive X and its transcriptional silencing. In an approach aimed at identifying genes implicated in the X-inactivation process by comparative transcriptional profiling of female and male mouse gastrula, we identified the Eif1 gene involved in translation initiation and RNA degradation. We show here that female embryonic stem cell lines, silenced by RNA interference for the Eif1 gene, are unable to form Xist RNA domains upon differentiation and fail to undergo X-inactivat

SUBMITTER: Ciaudo C 

PROVIDER: S-EPMC1479048 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature