Cytokine profiles of filarial granulomas in jirds infected with Brugia pahangi.

ABSTRACT:

Background

A granulomatous inflammatory response develops in jirds infected subcutaneously or intraperitoneally with filarial nematodes namely Brugia pahangi and B. malayi. Previous studies by light and electron microscopy have shown cellular inflammatory responses in and around these granulomas. Furthermore, the cellular inflammatory responses of granulomas found in the lymphatics and peritoneal cavity appear to be similar. The purpose of this study was to determine the cytokine profiles of granulomas in the peritoneal cavity of B. pahangi-infected jirds and to determine whether the granulomas release any proinflammatory cytokines ex vivo.

Methods

A semiautomated quantitative polymerase chain reaction (Q-PCR) was performed on cDNA prepared from the granulomas of infected ji

SUBMITTER: Rao RU 

PROVIDER: S-EPMC1479342 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature