Quantitative evaluation of RASSF1A methylation in the non-lesional, regenerative and neoplastic liver.

ABSTRACT:

Background

Epigenetic changes during ageing and their relationship with cancer are under the focus of intense research. RASSF1A and NORE1A are novel genes acting in concert in the proapoptotic pathway of the RAS signalling. While NORE1A has not been previously investigated in the human liver, recent reports have suggested that RASSF1A is frequently epigenetically methylated not only in HCC but also in the cirrhotic liver.

Methods

To address whether epigenetic changes take place in connection to age and/or to the underlying disease, we investigated RASSF1A and NORE1A gene promoter methylation by conventional methylation specific PCR and Real-Time MSP in a series of hepatitic and non-hepatitic livers harboring regenerative/hyperplastic (cirrhosis/focal nodular hyperplasia), dy

SUBMITTER: Di Gioia S 

PROVIDER: S-EPMC1479360 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature